Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1519, Issue -, Pages 137-144Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.08.084
Keywords
Capillary electrophoresis; Complex samples; Sample treatment; Supported liquid membrane; Simultaneous extraction
Funding
- Czech Academy of Sciences [RVO:68081715]
- Grant Agency of the Czech Republic [16-09135S]
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Simultaneous extraction of basic and acidic drugs across thin supported liquid membrane (SLM) and direct injection of the extracted drugs from SLM surface into capillary electrophoresis (CE) were demonstrated. A micro extraction device compatible with injection system of commercial CE instrument was filled with 20 mu L of sample and 10 mu L of acceptor solution, which were interspaced by the SLM impregnated with 5 mu L of organic solvent. Extractions of three basic drugs (nortriptyline, haloperidol and loperamide) and two acidic drugs (ketoprofen and naproxen) were achieved at optimized conditions including 1-ethyl-2-nitrobenzene as SLM solvent, natural pH of sample solution, 2.5 mM NaOH acceptor solution and 30 min extraction time. The extracted drugs were directly injected into CE for separation and quantification in a background electrolyte solution consisting of 30 mM ammonium acetate adjusted to pH 4.2 with acetic acid. The entire analytical procedure including drugs extraction, injection, separation and quantification was automated in the CE instrument and the only manual procedures were SLM impregnation and filling the microextraction device with sample and acceptor solutions. The analytical method was suitable for simultaneous determination of basic and acidic drugs in undiluted human urine samples and was used for direct determination of naproxen in urine after oral administration of Nalgesin S tablet. Efficient elimination of sample matrix and selective transfer of basic and acidic drugs were achieved and the hyphenated SLM-CE method was characterized by repeatability of peak areas ranging from 3.7 to 13.4%, linear relationship between peak areas and concentrations (r(2) =0.994-0.999) and limits of detection between 0.05 and 1.5 mu g/mL. (C) 2017 Elsevier B.V. All rights reserved.
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