Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1515, Issue -, Pages 37-44Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.07.092
Keywords
Bile salt; Microwave-assisted micellar extraction; Ultrasonic-assisted micellar extraction; Ultra-high performance liquid chromatography; Rhizoma zingiberis; Rhizoma zingiberis preparata
Funding
- Zhejiang Provincial Natural Science Foundation of China [LY15H280016]
- National Natural Science Foundation of China [81573552]
- Hangzhou social development of scientific research projects [20150533805]
- Program for Innovative Research Team in Chinese University [IRT 1231]
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Two kinds of extraction methods ultrasonic-assisted micellar extraction (UAME) and microwave-assisted micellar extraction (MAME) coupled with ultra-high performance liquid chromatography with ultraviolet detector (UHPLC-UV) were developed and evaluated for extraction and determination of zingerone, 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol in Rhizoma Zingiberis and Rhizoma Zingiberis Preparata. A biosurfactant, hyodeoxycholic acid sodium salt, was used in micellar extraction. Several experimental parameters were studied separately by a univariate method. The result indicated that the MAME was more efficient than UAME. The optimal conditions of MAME were as follows: 100 mM of hyodeoxycholic acid sodium salt was used as surfactant, the irradiation time was set at 10 s and the extraction temperature was set at 60 degrees C. The validation results indicated that the limits of detection were in the range of 3.80-8.11 ng/mL. The average recoveries were in the range of 87.32-103.12% for the two samples at two spiking levels. Compared with other reported methods, the proposed MAME-UHPLC-UV method was more effective, quicker (10 s) and more eco-friendly. (C) 2017 Elsevier B.V. All rights reserved.
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