Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1491, Issue -, Pages 153-158Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2017.02.059
Keywords
Centrifugal partition chromatography; Vitamin E; Deep eutectic solvents; Tocopherols; Biphasic solvent system screening; COSMO-RS
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Tocopherols are a class of molecules with vitamin E activity. Among those, alpha-tocopherol is the most important vitamin E source in the human diet. The purification of tocopherols involving biphasic liquid systems can be challenging since these vitamins are poorly soluble in water. Deep eutectic solvents (DES) can be used to form water-free biphasic systems and have already proven applicable for centrifugal partition chromatography separations. In this work, a computational solvent system screening was performed using the predictive thermodynamic model COSMO-RS. Liquid-liquid equilibria of solvent systems composed of alkanes, alcohols and DES, as well as partition coefficients of a-tocopherol, beta-tocopherol, gamma-tocopherol, and sigma-tocopherol in these biphasic solvent systems were calculated. From the results the best suited biphasic solvent system, namely heptane/ethanol/choline chloride-1,4-butanediol, was chosen and a batch injection of a tocopherol mixture, mainly consisting of alpha- and gamma-tocopherol, was performed using a centrifugal partition chromatography set up (SCPE 250-BIO). A separation factor of 1.74 was achieved for alpha- and gamma-tocopherol. (C) 2017 Elsevier B.V. All rights reserved.
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