Journal
JOURNAL OF CELLULAR PHYSIOLOGY
Volume 232, Issue 9, Pages 2387-2395Publisher
WILEY
DOI: 10.1002/jcp.25751
Keywords
Alzheimer's disease; buccal cells; super-resolution microscopy
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Funding
- Canada Foundation for Innovation
- Cognition Challenge Award (Johnson and Johnson Innovation)
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The advent of super-resolution microscopy allowed for new insights into cellular and physiological processes of normal and diseased cells. In this study, we report for the first time on the super-resolved DNA structure of buccal cells from patients with Alzheimer's disease (AD) versus age-and gender-matched healthy, non-caregiver controls. In this super-resolution study cohort of 74 participants, buccal cells were collected and their spatial DNA organization in the nucleus examined by 3D Structured Illumination Microscopy (3D-SIM). Quantitation of the super-resolution DNA structure revealed that the nuclear super-resolution DNA structure of individuals with AD significantly differs from that of their controls (p < 0.05) with an overall increase in the measured DNA-free/poor spaces. This represents a significant increase in the interchromatin compartment. Wealso find that theDNAstructure ofADsignificantly differs in mild, moderate, and severe disease with respect to the DNA-containing and DNA-free/poor spaces. We conclude that whole genome remodeling is a feature of buccal cells in AD.
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