Journal
JOURNAL OF CELL SCIENCE
Volume 131, Issue 3, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.207522
Keywords
Peroxisome; Organelle inheritance; Yeast; Replicative lifespan; Organelle; Peroxisome inheritance
Categories
Funding
- Netherlands Organization for Scientific Research (Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- NWO) [723.013.004]
- Marie Curie Initial Training Network (European Commission) PERFUME (PERoxisome Formation, Function, Metabolism) [316723]
Ask authors/readers for more resources
Here, we used fluorescence microscopy and a peroxisome-targeted tandem fluorescent protein timer to determine the relative age of peroxisomes in yeast. Our data indicate that yeast cells contain a heterogeneous population of relatively old and young peroxisomes. During budding, the peroxisome retention factor inheritance of peroxisomes protein 1 (Inp1) selectively associates to the older organelles, which are retained in the mother cells. Inp2, a protein required for transport of peroxisomes to the bud, preferentially associates to younger organelles. Using a microfluidics device, we demonstrate that the selective segregation of younger peroxisomes to the buds is carefully maintained during multiple budding events. The replicative lifespan of mother cells increased upon deletion of INP2, which resulted in the retention of all organelles in mother cells. These data suggest that, in wild-type yeast, transport of aged and deteriorated peroxisomes to the bud is prevented, whereas the young and vital organelles are preferably transported to the newly forming buds.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available