Journal
JOURNAL OF CELL SCIENCE
Volume 130, Issue 4, Pages 791-804Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.187914
Keywords
Peroxisomes; Pex35; Ygr168c; Arf1; High-content screen; Yeast
Categories
Funding
- State of Lower-Saxony, Hannover, Germany [ZN2921]
- European Research Council [CoG 646604]
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Peroxisomes are cellular organelles with vital functions in lipid, amino acid and redox metabolism. The cellular formation and dynamics of peroxisomes are governed by PEX genes; however, the regulation of peroxisome abundance is still poorly understood. Here, we use a high-content microscopy screen in Saccharomyces cerevisiae to identify new regulators of peroxisome size and abundance. Our screen led to the identification of a previously uncharacterized gene, which we term PEX35, which affects peroxisome abundance. PEX35 encodes a peroxisomal membrane protein, a remote homolog to several curvature-generating human proteins. We systematically characterized the genetic and physical interactome as well as the metabolome of mutants in PEX35, and we found that Pex35 functionally interacts with the vesicle-budding-inducer Arf1. Our results highlight the functional interaction between peroxisomes and the secretory pathway.
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