Journal
JOURNAL OF CELL BIOLOGY
Volume 217, Issue 1, Pages 179-193Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201612147
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Funding
- Wellcome Trust Senior Fellowship
- Medical Research Council
- Wellcome Trust [092096]
- Cancer Research UK [C6946/A14492]
- MRC [G1000818, G0701184] Funding Source: UKRI
- Medical Research Council [G0701184, G1000818] Funding Source: researchfish
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There is remarkable redundancy between the Cyclin-Cdk complexes that comprise the cell cycle machinery. None of the mammalian A-, D-, or E-type cyclins are required in development until implantation, and only Cdk1 is essential for early cell divisions. Cyclin B1 is essential for development, but whether it is required for cell division is contentious. Here, we used a novel imaging approach to analyze Cyclin B1-null embryos from fertilization onward. We show that Cyclin B1(-/-) embryos arrest in G2 phase after just two divisions. This is the earliest arrest of any Cyclin known and places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. We reintroduced mutant proteins into this genetically null background to determine why Cyclin B1 is constantly exported from the nucleus. We found that Cyclin B1 must be exported from the nucleus for the cell to prevent premature entry to mitosis, and retaining Cyclin B1-Cdk1 at the plasma membrane precludes entry to mitosis.
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