Journal
JOURNAL OF CELL BIOLOGY
Volume 216, Issue 11, Pages 3521-3534Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201703144
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Funding
- National Institutes of Health [R01CA190858]
- New Jersey Commission on Cancer Research pre-doctoral fellowships
- National Institutes of Health Ruth L. Kirschstein National Research service award [T32 GM8339]
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The BLM gene product, BLM, is a RECQ helicase that is involved in DNA replication and repair of DNA double-strand breaks by the homologous recombination (HR) pathway. During HR, BLM has both pro-and anti-recombinogenic activities, either of which may contribute to maintenance of genomic integrity. We find that in cells expressing a mutant version of BRCA1, an essential HR factor, ablation of BLM rescues genomic integrity and cell survival in the presence of DNA double-strand breaks. Improved genomic integrity in these cells is linked to a substantial increase in the stability of RAD51 at DNA double-strand break sites and in the overall efficiency of HR. Ablation of BLM also rescues RAD51 foci and HR in cells lacking BRCA2 or XRCC2. These results indicate that the anti-recombinase activity of BLM is of general importance for normal retention of RAD51 at DNA break sites and regulation of HR.
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