Journal
JOURNAL OF CELL BIOLOGY
Volume 216, Issue 6, Pages 1543-1556Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201609095
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Funding
- National Institutes of Health [P01 GM103723, P30 CA008748]
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Efficient collective migration depends on a balance between contractility and cytoskeletal rearrangements, adhesion, and mechanical cell-cell communication, all controlled by GTPases of the RHO family. By comprehensive screening of guanine nucleotide exchange factors (GEFs) in human bronchial epithelial cell monolayers, we identified GEFs that are required for collective migration at large, such as SOS1 and beta-PIX, and RHOA GEFs that are implicated in intercellular communication. Down-regulation of the latter GEFs differentially enhanced front-to-back propagation of guidance cues through the monolayer and was mirrored by down-regulation of RHOA expression and myosin II activity. Phenotype-based clustering of knockdown behaviors identified RHOA-ARH GEF18 and ARH GEF3-ARH GEF28-ARHGEF11 clusters, indicating that the latter may signal through other RHO-family GTPases. Indeed, knockdown of RHOC produced an intermediate between the two phenotypes. We conclude that for effective collective migration, the RHOA-GEFs. RHOA/C. actomyosin pathways must be optimally tuned to compromise between generation of motility forces and restriction of intercellular communication.
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