RHAMM regulates the growth and migration of lung adenocarcinoma A549 cell line by regulating Cdc2/CyclinB1 and MMP9 genes

Objective: The study aims to explore the effects of receptor of hyaluronan mediated motility (RHAMM) on the proliferation, invasion and migration of the lung adenocarcinoma (LUAD) cell line A549 and its targeted regulatory pathway. Methods: Bioinformatics was used to analyze the differentially expressed genes in LUAD chips. The mRNA and protein expression level of Cdc2, CyclinB1, MMPs and epithelial-mesenchymal transition (EMT) related markers E-cadherin and Vimentin were tested by qRT-PCR and western blot in A549 cell line after silencing RHAMM. Cell proliferation, cell division cycle, migration and invasion abilities were tested in RHAMM knockdown A549 cells by flow cytometry and in vitro assays. Results: Silencing RHAMM inhibited EMT, proliferation, migration and invasion of A549 cell line and induced cells to cluster at G2/M phase. In addition, after silencing RHAMM, the mRNA and protein expressions of Cdc2 and CyclinB1 were decreased while those of MMP9 were increased. Conclusion: The findings suggest that RHAMM regulates cell division cycle by regulating Cdc2 and CyclinB1, and regulates extracellular matrix degradation by regulating MMP9. These targeted modulations regulate the occurrence and development of LUAD cells.