Journal
JOURNAL OF BIOTECHNOLOGY
Volume 259, Issue -, Pages 228-234Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2017.06.1204
Keywords
CRISPR/Cas9 system; Polyketide synthase; Hypocrellin; Shiraia bambusicola; Virulence
Categories
Funding
- National Science Foundation of China [21275066]
- Six talent peak program [SWYY-126]
- Fundamental Research of Doctor of Philosophy [2050205, 205020502]
Ask authors/readers for more resources
Shiraia bambusicola can produce a type of hypocrellin, which is applied in antibacterial, antitumoral, and antiviral areas. Studies on the hypocrellin pathway have not been confirmed due to the deficiency of suitable genetic methods. We constructed a clustered regularly interspaced short palindromic repeat sequences (CRISPR)/Cas9 system in Shiraia sp. SUPER-H168 and targeted a polyketide synthase (SbaPKS). No hypocrellin production was detected in the ASbaPKS mutant. Relative expression levels of SbaPKS and its adjacent genes were extremely down-regulated in the dSbaPKS mutant compared to those in the wild strain. Subsequent pathogenicity assays showed that deletion of SbaPKS attenuated virulence on bamboo leaves. In contrast, restored hypocrellin in a SbaPKS overexpression strain generated necrotic lesions on bamboo leaves. These results suggest that SbaPKS is involved in hypocrellin biosynthesis and hypocrellin has an essential role in the virulence of S. bambusicola on bamboo leaves. The CRISPR/Cas9 system in Shiraia sp. will open an avenue for decoding the hypocrellin pathway and genome editing of other filamentous fungi. Strategies that disrupt hypocrellin biosynthesis may reduce the detriment of S. bambusicola.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available