Journal
JOURNAL OF BIOTECHNOLOGY
Volume 241, Issue -, Pages 33-41Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2016.11.006
Keywords
Amine dehydrogenase; Polyethylenimine; Hybrid immobilization; Thermostability; Bioinspired mineralization; Multimeric enzyme
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Funding
- National Natural Science Foundation of China [41176111, 41306124]
- State Key Program of National Natural Science Foundation of China [21336009]
- Fundamental Research Funds for the Central Universities [2013121029]
- Foundation of South Oceanographic Research Center of China in Xiamen [14GYY011NF11]
- Public science and technology research funds projects of ocean [201505032-6]
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Asymmetric synthesis of chiral amines by amine dehydrogenases (AmDH), which catalyzed reductive amination of ketones with high enantioselectivity, is an ideal route for production of chiral amine. In this study, a facile approach is proposed to immobilize unstable amine dehydrogenase by two steps. Firstly, polyethylenimine (PEI), a cationic polymer, was applied for coating enzyme to inhibit the dissociation of multimeric enzymes. Strong interaction of PEI with AmDH were analyzed and indicated PEI provided the hydrophilic microenvironment for enzyme. The half-life was improved about 18 fold in 50 degrees C. Secondly, to further stabilize AmDH-PEI, PEI coated on AmDH surface Was used as a biological template inducing the hydrolysis and condensation of titanium precursor to form titania. AmDH-PEI-Ti possessed more than 80% of the catalytic activity of free enzyme and the entrapment efficiency can be high up to 90%. A mechanistic illustration of the formation of AmDH-PEI-Ti nanoparticles were proposed. Titania provided a rigid cage pocket for the protection from structure unfolding. This generally applicable strategy offers a potential technique for multimeric enzyme immobilization with the advantages of low cost, easy operation, high reservation of activity and high stability. (C) 2016 Elsevier B.V. All rights reserved.
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