Journal
JOURNAL OF BIOTECHNOLOGY
Volume 261, Issue -, Pages 10-23Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2017.08.012
Keywords
Metagenomics; High-throughput 16S rRNA gene amplicon sequencing; Next-generation sequencing; Biogas; Anaerobic digestion; de.NBI
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Funding
- German Federal Ministry of Education and Research (BMBF) of the project 'Bielefeld-Giessen Center for Microbial Bioinformatics - BiGi' within the German Network for Bioinformatics Infrastructure (de.NBI) [031A533]
- German Federal Ministery of Food and Agriculture (BMEL) via the Fachagentur fur Nachwachsende Rohstoffe e.V. (FNR) of the joint research project Biogas-Core [FKZ 22017111]
- German Federal Ministery of Food and Agriculture (BMEL) via the Fachagentur fur Nachwachsende Rohstoffe e.V. (FNR) of the joint research project BMP-III [FKZ 22404015]
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Metagenomics has proven to be one of the most important research fields for microbial ecology during the last decade. Starting from 16S rRNA marker gene analysis for the characterization of community compositions to whole metagenome shotgun sequencing which additionally allows for functional analysis, metagenomics has been applied in a wide spectrum of research areas. The cost reduction paired with the increase in the amount of data due to the advent of next-generation sequencing led to a rapidly growing demand for bioinformatic software in metagenomics. By now, a large number of tools that can be used to analyze metagenomic datasets has been developed. The Bielefeld-Gie beta en center for microbial bioinformatics as part of the German Network for Bioinformatics Infrastructure bundles and imparts expert knowledge in the analysis of metagenomic datasets, especially in research on microbial communities involved in anaerobic digestion residing in biogas reactors. In this review, we give an overview of the field of metagenomics, introduce into important bioinformatic tools and possible workflows, accompanied by application examples of biogas surveys successfully conducted at the Center for Biotechnology of Bielefeld University.
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