Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 123, Issue 1, Pages 109-115Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2016.07.018
Keywords
Biocatalysis; Escherichia coli; trans-4-Hydroxy-L-proline; Vitreoscilla hemoglobin; Fermentation
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trans-4-Hydroxy-L-proline (Hyp) is a chiral amino acid conventionally produced by acid hydrolysis of animal collagen, a process which involves the bottleneck problems of low efficiency and heavy environmental pollution. Biotransformation of L-proline into Hyp using recombinant whole-cell biocatalysis with proline-4-hydroxylase (P4H) is an environmentally-friendly alternative method. Since biohydroxylation of proline by whole cells is a high-oxygen-demand process, oxygen transfer needs to be improved. To solve this problem, the Vitreoscilla hemoglobin gene (vgb) was integrated into the chromosome of recombinant Escherichia coli expressing the P4H gene originally from Dactylosporangium sp. RH1. Expression of Vitreoscilla hemoglobin (VHb) resulted in a 94.4% increase of Hyp production in a 100-mL shaking flask culture compared to the same strain without VHb expression. Meanwhile in a fed-batch fermentation with a 1.4 L bioreactor, the expression of VHb led to an increase in Hyp production by 73.2% and biomass improved by 106%. We also found that acetic acid concentration was decreased by the expression of VHb during the fermentation. This work demonstrates that vgb chromosomal integration is an efficient way to improve Hyp production by enhancing oxygen transfer in recombinant E. coli. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
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