Journal
CHEMICAL COMMUNICATIONS
Volume 56, Issue 16, Pages 2423-2426Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9cc09594b
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Funding
- National Natural Science Foundation of China [21625501, 21527808]
- Beijing Outstanding Young Scientist Program [BJJWZYJH01201910005017]
- Beijing Municipal High Level Innovative Team Building Program [IDHT20180504]
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A highly sensitive laser-induced fluorescence detection system with real-time imaging focusing instead of the use of fluorescent reagents were developed for the detection of analytes in nanocapillaries. A cylindrical lens was adopted for the shaping of a laser beam to increase its spatial resolution. The limit of detection with a capillary having a radius of 440 nm was 6.76 yoctomoles (or four molecules) for fluorescein, and 84.5 yoctomoles (or 51 molecules) for FAM-labeled Hsa-miR-17. The designed system exhibited high sensitivity and stability for femtoliters of sample and was hence indicated to be suitable for analyses of single cells.
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