Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 22, Issue 4, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.22.4.046009
Keywords
second-harmonic generation imaging; posterior cruciate ligament; crimp analysis; Fourier transform analysis
Funding
- University of Illinois at Urbana-Champaign
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We use second-harmonic generation (SHG) microscopy to quantitatively characterize collagen fiber crimping in the posterior cruciate ligament (PCL). The obtained SHG images are utilized to define three distinct categories of crimp organization in the PCL. Using our previously published spatial-frequency analysis, we develop a simple algorithm to quantitatively distinguish the various crimp patterns. In addition, SHG microscopy reveals both the three-dimensional structural variation in some PCL crimp patterns as well as an underlying helicity in these patterns that have mainly been observed using electron microscopy. Our work highlights how SHG microscopy could potentially be used to link the fibrous structural information in the PCL to its mechanical properties. (C) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
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