Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Volume 106, Issue 8, Pages 2741-2749Publisher
WILEY
DOI: 10.1002/jbm.b.34055
Keywords
hydrogel; bacterial nanocellulose; IKVAV; vasculogenic mimicry; human melanoma cells
Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [402901/2013-4, 486753/2012-3, 550009/2014-0, 550084/2014-2]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [1434128]
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Vasculogenic mimicry process has generated great interest over the past decade. So far, however, there have been only a few matrices available that allow us to study that process in vitro. Here, we have developed an innovative hydrogel platform with defined composition that mimics the structural architecture and biological functions of the extracellular matrix for vasculogenic mimicry of human melanoma cells (SK-MEL-28). We chemically immobilized IKVAV peptide on bacterial nanocellulose (BNC) fibers. BNC-IKVAV hydrogel was found to improve the adhesion and proliferation of SK-MEL-28 cells on the top and bottom surfaces. Particularly, the bottom surface of BNC-IKVAV induced SK-MEL-28 cells to organize themselves as well-established networks related to the vasculogenic mimicry process. Finally, our results showed that not only BNC-IKVAV but also BNC hydrogels can potentially be used as a three-dimensional platform that allows the screening of antitumor drugs. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2741-2749, 2018.
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