Journal
CHEMICAL SCIENCE
Volume 11, Issue 10, Pages 2657-2663Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9sc05976h
Keywords
-
Categories
Funding
- CNRS
- ENSCP
- French Ministry of Research and Higher Education
- ERC Consolidator Grant PhotoMedMet [GA 681679]
- ANR [ANR-10-IDEX-0001-02 PSL]
- Institut Pasteur
- Canceropole Ile-de-France
- ARC Foundation for Cancer Research [S-FB18006]
- Ile-de-France Region
- IPGP multidisciplinary program PARI
- Region Ile-de-France SESAME Grant [12015908]
Ask authors/readers for more resources
Ruthenium complexes have attracted a lot of attention as potential photosensitizers (PSs) for photodynamic therapy (PDT). However, some of these PSs are unsuitable for PDT applications due to their low cellular uptake, which is possibly the consequence of their relatively low degree of lipophilicity, which prevents them from penetrating into tumor cells. Here, we report the simple one-pot synthesis of ruthenium-containing nanoconjugates from a non-cell-penetrating, non-phototoxic ruthenium(ii) polypyridyl complex (RuOH), by a drug-initiated ring-opening polymerization of lactide through the formation of a zinc initiator. These conjugates were then formulated into nanoparticles by nanoprecipitation and characterized by means of nuclear magnetic resonance spectroscopy (NMR), matrix-assisted laser desorption/ionization - time of flight mass spectrometry (MALDI-TOF MS) and dynamic light scattering (DLS). Finally, their photo-therapeutic activity (lambda(exc) = 480 nm, 3.21 J cm(-2)) in cancerous human cervical carcinoma (HeLa) and non-cancerous retinal pigment epithelium (RPE-1) cells was tested alongside that of RuOH and their cellular uptake in HeLa cells was assessed by confocal microscopy and inductively coupled plasma - mass spectrometry (ICP-MS). All nanoparticles showed improved photophysical properties including luminescence and singlet oxygen generation, enhanced cellular uptake and, capitalizing on this, an improved photo-toxicity. Overall, this study demonstrates how it is possible to transform a non-phototoxic PDT PS into an active PS using an easy, versatile polymerization technique.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available