Journal
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Volume 28, Issue 9, Pages 864-878Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2017.1311821
Keywords
Poly (lactide-co-glycolide); polycaprolactone; biphasic calcium phosphate; bone regeneration
Funding
- Vietnam National University - Ho Chi Minh City [1161/ QD-DHQG-KHCN]
- Office of Navy Research (ONR) [N62909-14-1-N011-P00001]
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In this study, biomimic porous polycaprolactone/poly (lactide-co-glycolide) loading biphasic tricalcium phosphate (PCL/PLGA-BCP) scaffolds were fabricated successfully by solvent evaporation method. The distribution of biphasic tricalcium phosphate (BCP) in polycaprolactone/poly (lactide-co-glycolide) (PCL/PLGA) scaffold was confirmed by micro-computed tomography (micro-CT) scanning, scanning electron microscope (SEM) observation and Energy-dispersive X-ray Spectroscopy (EDS) analysis. The hydrophilicity of the scaffolds was confirmed by contact angle measurement. In in vitro experiments, proliferation of human bone marrow mesenchymal stem cell (hBMSCs) and its osteoblastic differentiation on scaffold were assessed for 1, 2 and 3weeks using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, fluorescence observation, hematoxylin & eosin (H&E) staining and real-time polymerase chain reaction (RT-PCR). In in vivo experiments, ossification was observed using micro-CT analysis and histological staining.
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