Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 292, Issue 18, Pages 7452-7461Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M116.771667
Keywords
E3 ubiquitin ligase; inflammation; lung; lung injury; ubiquitin
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Funding
- United States Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development, Biomedical Laboratory Research and Development
- United States Department of Veterans Affairs
- Flight Attendant Medical Research Institute
- National Institutes of Health R01 Grants [HL096376, HL097376, HL098174, HL081784, 1UH2HL123502, P01HL114453, R01 HL131665, R01 GM115389]
- American Heart Association GIA award
- American Lung Association [BRG RG350146]
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Glycogen synthase kinase-3 (GSK3) has diverse biological roles including effects on cellular differentiation, migration, and inflammation. GSK3 phosphorylates proteins to generate phosphodegrons necessary for recognition by Skp1/Cullin-1/F-box (SCF) E3 ubiquitin ligases leading to subsequent proteasomal degradation of these substrates. However, little is known regarding how GSK3 protein stability itself is regulated and how its stability may influence inflammation. Here we show that GSK3 is degraded by the ubiquitin-proteasome pathway in murine lung epithelial cells through lysine 183 as an acceptor site for K48 polyubiquitination. We have identified FBXO17 as an F-box protein subunit that recognizes and mediates GSK3 polyubiquitination. Both endogenous and ectopically expressed FBXO17 associate with GSK3, and its overexpression leads to decreased protein levels of GSK3. Silencing FBXO17 gene expression increased the half-life of GSK3 in cells. Furthermore, overexpression of FBXO17 inhibits agonist-induced release of keratinocyte-derived cytokine (KC) and interleukin-6 (IL-6) production by cells. Thus, the SCFFBXO17 E3 ubiquitin ligase complex negatively regulates inflammation by targeting GSK3 in lung epithelia.
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