Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 292, Issue 18, Pages 7462-7473Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M117.780155
Keywords
adaptor protein; crystal structure; dynein; intracellular trafficking; primary cilium; IFT; cilium; flagellum; intraflagellar transport; outer dynein arms
Categories
Funding
- Novo Nordisk Foundation [NNF15OC0014164]
- European Research Council [310343]
- Novo Nordisk Fonden [NNF15OC0016050] Funding Source: researchfish
- European Research Council (ERC) [310343] Funding Source: European Research Council (ERC)
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Motile cilia are found on unicellular organisms such as the green alga Chlamydomonas reinhardtii, on sperm cells, and on cells that line the trachea and fallopian tubes in mammals. The motility of cilia relies on a number of large protein complexes including the force-generating outer dynein arms (ODAs). The transport of ODAs into cilia has been previously shown to require the transport adaptor ODA16, as well as the intraflagellar transport (IFT) protein IFT46, but the molecular mechanism by which ODAs are recognized and transported into motile cilia is still unclear. Here, we determined the high-resolution crystal structure of C. reinhardtii ODA16 (CrODA16) and mapped the binding to IFT46 and ODAs. The CrODA16 structure revealed a small 80-residue N-terminal domain and a C-terminal 8-bladed -propeller domain that are both required for the association with the N-terminal 147 residues of IFT46. The dissociation constant of the IFT46-ODA16 complex was 200 nm, demonstrating that CrODA16 associates with the IFT complex with an affinity comparable with that of the individual IFT subunits. Furthermore, we show, using ODAs extracted from the axonemes of C. reinhardtii, that the C-terminal -propeller but not the N-terminal domain of CrODA16 is required for the interaction with ODAs. These data allowed us to present an architectural model for ODA16-mediated IFT of ODAs.
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