Journal
LAB ON A CHIP
Volume 20, Issue 6, Pages 1103-1109Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9lc00979e
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Funding
- Ministry of Science and Technology (MOST) of Taiwan [MOST 108-2314-B-007-002, MOST 107-2221-E-007-013-MY3]
- Higher Education Sprout Project of Taiwan's Ministry of Education [108Q2713E1]
- National Health Research Institutes [NHRI-EX107-10728EI]
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Accurate cancer diagnostic methods are of urgent need. Since traditional immunohistochemistry (IHC)-based approaches, while reliable, are labor-intensive and require well-trained technicians, we developed an integrated microfluidic platform capable of labeling ovarian cancer biomarkers (i.e. aptamer) within formalin-fixed, paraffin embedded tissues via molecular probes. Both aptamer-based 1) fluorescent staining and 2) IHC staining of clinical tissue samples could be automated in the microfluidic system in only 2-3 h (40-50% faster than conventional approaches) with <0.5 mL of reagents, signifying that this device could serve as a promising diagnostic tool for ovarian cancer.
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