Transforming Growth Factor-β (TGF-β) Directly Activates the JAK1-STAT3 Axis to Induce Hepatic Fibrosis in Coordination with the SMAD Pathway

Transforming growth factor-beta (TGF-beta) signals through both SMAD and non-SMAD pathways to elicit a wide array of biological effects. Existing data have shown the association and coordination between STATs and SMAD sin mediating TGF-beta functions in hepatic cells, but it is not clear how STATs are activated under these circumstances. Here, we report that JAK1 is a constitutive TGF beta RI binding protein and is absolutely required for phosphorylation of STATs in a SMAD-independent manner within minutes of TGF-beta stimulation. Following the activation of SMADs, TGF-beta also induces a second phase of STAT phosphorylation that requires SMADs, de novo protein synthesis, and contribution from JAK1. Our global gene expression profiling indicates that the non-SMAD JAK1/STAT pathway is essential for the expression of a subset of TGF-beta target genes in hepatic stellate cells, and the cooperation between the JAK1STAT3 and SMAD pathways is critical to the roles of TGF-beta in liver fibrosis.