Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 293, Issue 5, Pages 1820-1834Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M117.814152
Keywords
directed evolution; major histocompatibility complex (MHC); protein engineering; protein-protein interaction; T-cell receptor (TCR); deep sequencing; single-codon libraries; yeast display
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Funding
- National Institutes of Health [CA178844, CA187592]
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Most affinity-maturation campaigns for antibodies and T-cell receptors (TCRs) operate on the residues at the binding site, located within the loops known as complementarity-determining regions (CDRs). Accordingly, mutations in contact residues, or so-called second shell residues, that increase affinity are typically identified by directed evolution involving combinatorial libraries. To determine the impact of residues located at a distance from the binding site, here we used single-codon libraries of both CDR and non-CDR residues to generate a deep mutational scan of a human TCR against the cancer antigen MART-1HLA-A2. Non-CDR residues included those at the interface of the TCR variable domains (V and V) and surface-exposed framework residues. Mutational analyses showed that both V/V interface and CDR residues were important in maintaining binding to MART-1HLA-A2, probably due to either structural requirements for proper V/V association or direct contact with the ligand. More surprisingly, many V/V interface substitutions yielded improved binding to MART-1HLA-A2. To further explore this finding, we constructed interface libraries and selected them for improved stability or affinity. Among the variants identified, one conservative substitution (F45Y) was most prevalent. Further analysis of F45Y showed that it enhanced thermostability and increased affinity by 60-fold. Thus, introducing a single hydroxyl group at the V/V interface, at a significant distance from the TCRpeptideMHC-binding site, remarkably affected ligand binding. The variant retained a high degree of specificity for MART-1HLA-A2, indicating that our approach provides a general strategy for engineering improvements in either soluble or cell-based TCRs for therapeutic purposes.
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