Immobilization of acetylcholinesterase on Pt(II) and Pt(IV) attached nanoparticles for the determination of pesticides

Pt(II) and Pt(IV)-tagged nanoparticles have been synthesized according to the template method for the identification of pesticides. Their morphologies have been investigated using scanning electron microscopy and characterized by means of spectral measurements. Then, acetylcholinesterase (AChE) was immobilized onto the nanoparticles. The AChE immobilized Pt(II) and Pt(IV)-tagged nanomaterials show high reusability and storage capacity. The catalytic activity of AChE followed Michaelis-Menten kinetics. Assays for enzyme activity measurements demonstrate that the nanospheres tagged with Pt(II) have a much better performance than those with Pt(IV). Furthermore, whether or not there was any interaction between the immobilized enzyme and 1-naphthyl-N-methylcarbamate, which is a carbamate insecticide, was examined.