Journal
ACTA CHROMATOGRAPHICA
Volume 32, Issue 2, Pages 134-138Publisher
AKADEMIAI KIADO ZRT
DOI: 10.1556/1326.2019.00611
Keywords
HPLC; core shell column; phenolics; wine
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Funding
- National Project of Technological Development - Ministry of Education, Science and Technological Development, Republic of Serbia [TP31020]
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Phenolic compounds are frequently present in various natural products, and they can have different beneficial biological potentials. The most widely used method for determination of individual phenolic compounds is high-performance liquid chromatography (HPLC). In this paper, a method simultaneous determination of 16 phenolic compounds (gallic acid, p-hydroxyhenzoic acid, catechin, syringic acid, trans-cinnamic acid, hesperetin, naringenin, vanillic acid, benzoic acid, coumaric acid, resveratrol, chlorogenic acid, caffeic acid, rutin, quercetin, and kaempferol) on a core shell column was developed. The separation method conducted on a standard ODS (250 mm) column was transferred to Poroshell column and optimized using non-ultra-high-performance liquid chromatography (UHPLC) apparatus. Phenolic compounds were separated fast and efficiently during 30-min analysis, and validation parameters were determined. The developed method was successfully applied on the analysis of phenolic content after direct injection of red wines from three different grape varieties.
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