Journal
BIOSENSORS-BASEL
Volume 10, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/bios10030018
Keywords
passive microfluidic device; cell culture; micro-bioreactor
Funding
- Italian Ministry of Health's 'High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices' [CUP J65C13001350001, GR-2010-2311677]
- European Commission
- FESR FES 2014-2020
- Calabria Region
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In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 mu L/h - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 h. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 h.
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