Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 29, Issue 3, Pages 1623-1633Publisher
SPRINGER
DOI: 10.1007/s10811-016-1037-7
Keywords
Macroalgae; Pyropia haitanensis; Rhodophyta hydrolysis; Anti-proliferation; Peptides
Funding
- Ocean and Fisheries Development Project from the Administration of Ocean and Fisheries of Guangdong Province [A201401C08]
- Guangdong Province Science and Technology plan project [2014A02020817]
- South China University of Technology Postdoctoral Fund [X2qsL1150040]
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In this study, proteins were extracted from the red macroalga Pyropia haitanensis and hydrolyzed with pepsin and papain. Gel filtration chromatography was performed to separate hydrolysates. Three fractions were obtained from papain-digested hydrolysates, which showed anti-proliferation effects on human breast cancer cells (MCF-7), human liver cancer cells (HepG-2), and human lung cancer cells (A549), with IC50 values of 59.09-272.67 mu g mL(-1). Furthermore, a new peptide QTDDNHSNVLWAGFSR was identified, which had the best inhibition on HepG-2 cancer cells (61.36%), but low inhibition on human normal liver cells LO-2 (22.81%), at 500 mu g mL(-1). This is the first time that anti-proliferative peptides originating from protein hydrolysates of P. haitanensis were isolated and characterized.
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