Journal
JOURNAL OF APPLIED ORAL SCIENCE
Volume 25, Issue 6, Pages 680-688Publisher
UNIV SAO PAULO FAC ODONTOLOGIA BAURU
DOI: 10.1590/1678-7757-2017-0050
Keywords
Flowability; Methylcellulose; MTA; Osteogenic differentiation
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Funding
- Chonnam National University Hospital Biomedical Research Institute [CRI 16025-1]
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2016R1C1B1012703]
- National Research Foundation of Korea [2016R1C1B1012703] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Objectives: Methylcellulose (MC) is a chemical compound derived from cellulose. MTA mixed with MC reduces setting time and increases plasticity. This study assessed the influence of MC as an anti-washout ingredient and CaCl2 as a setting time accelerator on the physical and biological properties of MTA. Material and Methods: Test materials were divided into 3 groups; Group 1(control): distilled water; Group 2:1% MC/CaCl2; Group 3:2% MC/CaCl2. Compressive strength, pH, flowability and cell viability were tested. The gene expression of bone sialoprotein (BSP) was detected by RT-PCR and real-time PCR. The expression of alkaline phosphatase (ALP) and mineralization behavior were evaluated using an ALP staining and an alizarin red staining. Results: Compressive strength, pH, and cell viability of MTA mixed with MC/CaCl2 were not significantly different compared to the control group. The flowability of MTA with MC/CaCl2 has decreased significantly when compared to the control (p<.05). The mRNA level of BSP has increased significantly in MTA with MC/CaCl2 compared to the control (p<.05). This study revealed higher expression of ALP and mineralization in cells exposed to MTA mixed with water and MTA mixed with MC/CaCl2 compared to the control (p<.05). Conclusions: MC decreased the flowability of MTA and did not interrupt the physical and biological effect of MTA. It suggests that these cements may be useful as a root-end filling material.
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