Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 122, Issue 3, Pages 785-795Publisher
WILEY
DOI: 10.1111/jam.13372
Keywords
comprehensive two-dimensional gas chromatography; headspace analysis; Klebsiella pneumoniae; mass spectrometry; metabolomics; volatile molecules
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Funding
- Burroughs Wellcome Fund
- Paul K. Richter and Evalyn E. Cook Richter Memorial Fund
- [1014106]
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Aims: The purpose of this study was to identify the volatile molecules produced by the pathogenic Gram-negative bacterium Klebsiella pneumoniae (ATCC 13883) during invitro growth using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GCxGC-TOFMS). Methods and Results: Klebsiella pneumoniaeATCC 13883 was incubated in lysogeny broth to mid-exponential and stationary growth phases. Headspace volatile molecules from culture supernatants were concentrated using solid-phase microextraction (SPME) and analysed via GCxGC-TOFMS. Ninety-two K. pneumoniae-associated volatile molecules were detected, of which 78 (85%) were detected at both phases of growth and 14 (15%) were detected at either mid-exponential or stationary growth phases. Conclusions: This study has increased the total number of reported K. pneumoniae-associated volatile molecules from 77 to 150, demonstrating the sensitivity and resolution achieved by employing GCxGC-TOFMS for the analysis of bacterial headspace volatiles. Significance and Impact of the Study: This study represents an early-stage comprehensive volatile metabolomic analysis of an opportunistic bacterial pathogen. Characterizing the volatile molecules produced by K. pneumoniae during invitro growth could provide us with a better understanding of this organisms' metabolism, an area that has not been extensively studied to date.
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