Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 123, Issue 5, Pages 1133-1147Publisher
WILEY
DOI: 10.1111/jam.13580
Keywords
antibiotics; bacteriocins; genes; identification; molecular genetic
Categories
Funding
- Military Biodefense Research Laboratory Program - Agency for Defense Development [FDC0941722]
- New Industry R&D program - Ministry of Trade, Industry and Energy [TGC0751614]
- KRIBB Research Initiative Program, Ministry of Science, ICT and Future Planning, Republic of Korea [KGM4531713]
- National Research Council of Science & Technology (NST), Republic of Korea [KGM4531713] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
AimsTo analyse the function of a putative lantibiotic gene cluster of Paenibacillus polymyxa E681 and to characterize its product, paenilan. Methods and ResultsComparative analysis of the lantibiotic gene cluster of E681 revealed that the cluster, consisting of 11 open reading frames, is involved in the biosynthesis of a class I lantibiotic. The pnlA gene encoding the prepeptide PnlA was identified and P. polymyxa strain EPT14 producing only paenilan was constructed by knockout of the other five antibiotic biosynthetic gene clusters of E681. Paenilan was purified from EPT14 culture by solvent partitioning, ODS silica gel chromatography and reversed-phase preparative HPLC. The molecular mass (251010Da) and structure of paenilan analysed by Nanoelectrospray ionization mass spectrometry (MS) and MS/MS showed that paenilan is a novel class I lantibiotic. Paenilan exhibited antimicrobial activity against Gram-positive bacteria such as Bacillus cereus, Micrococcus luteus and Paenibacillus durus. The paenilan gene is well-conserved in different Paenibacillus sp. isolated from globally distant places. ConclusionsThe lantibiotic gene cluster of P. polymyxa E681 was analysed and its product, a novel and useful lantibiotic named paenilan that inhibits the growth of some Gram-positive bacteria, was characterized. Significance and Impact of the StudyPaenibacillus species are a good source of new lantibiotics, and the conservation of the paenilan gene among Paenibacillus sp. implies paenilan has an important function(s) for their survival.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available