4.6 Article

Influence of l-amino acids on aggregation and biofilm formation in Azotobacter chroococcum and Trichoderma viride

Journal

JOURNAL OF APPLIED MICROBIOLOGY
Volume 123, Issue 4, Pages 977-991

Publisher

WILEY
DOI: 10.1111/jam.13534

Keywords

aggregation; ATR-FT-IR; biofilm; functional group analysis; l-amino acids; principal component analysis

Funding

  1. Post Graduate School, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute (IARI) (New Delhi, India)
  2. Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute (IARI) (New Delhi, India)
  3. ICAR-Application of Microorganisms in Agriculture and Allied Sectors (AMAAS) Network Project on Micro-organisms

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AimThe effects of l-amino acids on growth and biofilm formation in Azotobacter chroococcum (Az) and Trichoderma viride (Tv) as single (Az, Tv) and staggered inoculated cultures (Az-Tv, Tv-Az) were investigated. Methods and ResultsA preliminary study using a set of 20 l-amino acids, identified 6 amino acids (l-Glu, l-Gln, l-His, l-Ser, l-Thr and l-Trp) which significantly enhanced growth and biofilm formation. Supplementation of these amino acids at different concentrations revealed that 40mmoll(-1) was most effective. l-Glu and l-Gln favoured planktonic growth in both single and in staggered inoculated cultures, while l-Trp and l-Thr, enhanced aggregation and biofilm formation. Addition of l-Glu or l-Gln increased carbohydrate content and planktonic population. Principal component analysis revealed the significant role of proteins in growth and biofilm formation, particularly with supplementation of l-Trp, l-Thr and l-Ser. Azotobacter was found to function better as biofilm under staggered inoculated culture with Trichoderma. ConclusionsThe results illustrate that amino acids play crucial roles in microbial biofilm formation, by influencing growth, aggregation and carbohydrates synthesized. Significance and Impact of the StudyThe differential and specific roles of amino acids on biofilm formation are of significance for agriculturally important micro-organisms that grow as biofilms, colonize and benefit the plants more effectively.

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