Journal
VIRULENCE
Volume 11, Issue 1, Pages 327-336Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/21505594.2020.1747799
Keywords
E; coli; virulence; ExPEC; mouse sepsis model
Categories
Funding
- Instituto de Salud Carlos III of Spain [PI13/02092, CM18/00157]
- Spanish Network for Research in Infectious Diseases (REIPI) [RD12/0015/0004, RD16/0016/0011]
- Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC)
- European Development Regional Fund (ERDF)
- A Way to Achieve Europe
- REIPI [RD12/0015/0004, RD16/0016/0011]
- Office of Research and Development, Medical Research Service, Department of Veterans Affairs [1 I01 CX000192 01]
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Background: Escherichia coli ST131, mainly its H30 clade, is the leading cause of extraintestinal E. coli infections but its correlates of virulence are undefined. Materials and methods: We tested in a murine sepsis model 84 ST131 isolates that differed by country of origin (Spain vs. USA), clonal subset, resistance markers, and virulence genes (VGs). Virulence outcomes, including illness severity score (ISS) and killer status (>80% mouse lethality), were compared statistically with clonal subset, individual and combined VGs, molecularly defined extraintestinal and uropathogenic E. coli (ExPEC, UPEC) status, and country of origin. Results: Virulence varied widely by strain. Univariable correlates of median ISS and percent killer (outcomes if variable present vs. absent) included pap (ISS, 4.4 vs. 3.8; killer, 71% vs. 46%), kpsMII (4.1 vs. 2.3; 59% vs. 25%), K2/K100 (4.4 vs. 3.2; 77% vs. 41%), ExPEC (4.2 vs. 2.2; 62% vs. 17%), Spanish origin (4.3 vs. 3.1; 65% vs. 36%), and H30R1 subset (2.5 vs. 4.1; 35% vs. 59%). With multivariable adjustment, ExPEC status was the only consistently significantly predictive variable. Conclusion: Within ST131 the strongest predictor of experimental virulence was molecularly defined ExPEC status. Clonal subsets seemed to behave differently in the murine sepsis model by country of origin.
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