Journal
REPRODUCTION FERTILITY AND DEVELOPMENT
Volume 32, Issue 6, Pages 610-618Publisher
CSIRO PUBLISHING
DOI: 10.1071/RD19024
Keywords
AVPV; gonadotrophin-releasing hormone; hypothalamic-pituitary-gonadal axis; neuroendocrinological development; prepubertal exposure; reproductive function
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Funding
- Fujian Provincial Health and Family Planning Research Talents Training Project [2018-ZQN-21]
- Fujian Provincial Hospital [2017 GL-003]
- National Natural Science Foundation of China [81570706]
- Natural Science Foundation of China [81770848]
- Natural Science Foundation of Fujian Province [2016Y9006]
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Di-(2-ethylhexyl) phthalate (DEHP) is ubiquitous in the environment and has been proposed to lead to reproductive disruption. In this study, we systematically investigated the effects of different doses of DEHP exposure on female hypothalamic-pituitary-gonadal axis development. Female Sprague-Dawley rats were gavaged with vehicle (corn oil) or DEHP (5 or 500 mg kg(-1) day(-1)) during postnatal Days (PNDs) 22-28 or PNDs 22-70. Results demonstrated that the low and high doses of DEHP exerted opposite effects on puberty onset, circulating luteinising hormone, serum oestradiol and progesterone levels, with the low dose (5 mg kg(-1)) promoting and the high dose (500 mg kg(-1)) inhibiting these parameters. Significant dose-related differences were also found in the D500 group with longer oestrous cycle duration, lower ovarian/bodyweight ratio, fewer corpus lutea and more abnormal ovarian stromal tissue in comparison with the oil or D5 groups. Molecular data showed that the hypothalamic Kiss1 mRNA expression in the anteroventral periventricular but not in the arcuate nucleus significantly decreased in the D500 rats and increased in the D5 rats relative to the rats in the oil group. These findings suggested that the kisspeptin system is a potential target for DEHP to disrupt reproductive development and function.
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