Journal
ANALYST
Volume 145, Issue 8, Pages 3106-3115Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0an00224k
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Funding
- National Key Research and Development Program of China [2018YFC1603600]
- Jiangsu Agricultural Independent Innovation Project [SCX(18)2011]
- Central Guidance for Local Science and Technology Development [YDZX20173100004528]
- Science and Technology Joint Project of the Yangzte River Delta [17395810102]
- National 'Youth Top-notch Talent' Support Program [W0270187]
- Introduction of Nanjing Agricultural University Scientific Research Grants Project [804121]
- Jiangsu Collaborative Innovation Center of Meat Production and Processing
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Escherichia coli O157:H7 is a severe foodborne pathogen that causes lots of life-threatening diseases. In the search for a rapid, sensitive, portable and low-cost method to detect this pathogen, we developed a wax-printed paper-based enzyme-linked immunosorbent assay (P-ELISA) based on microfluidic paper-based analytical devices (mu PADs), with the whole operation time being less than 3 h and only needing 5 mu l samples for detection. The limit of detection (LOD) of E. coli O157:H7 reached 10(4) CFU ml(-1), which is an order of magnitude higher than that of conventional ELISA (C-ELISA). The LOD in artificially contaminated beef samples is 1 CFU per 25 g after enriching the culture for 8 h. This method is superior to the molecular biology method in detection sensitivity and superior to C-ELISA and the national standard method in detection time and cost. Thus, the established P-ELISA method has good sensitivity, specificity and repeatability. It can be suitable for point-of-care testing without expensive and bulky instruments and can also provide a platform for detecting other pathogens, especially in areas that lack advanced clinical equipment.
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