Journal
SUSTAINABLE ENERGY & FUELS
Volume 4, Issue 4, Pages 1963-1973Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9se00943d
Keywords
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Funding
- C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [NRF-2015M3D3A1A01064883]
- New and Renewable Energy Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grants from the Ministry of Trade, Industry and Energy [20173010092460]
- National Research Foundation of Korea [2015M3D3A1A01064883] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A packed-bed reactor (PBR) which has recyclable internal gas and medium exchange functions for carbon monoxide (CO) fermentation was operated using an ethanol producing acetogen, Clostridium autoethanogenum DSM 10061. The PBR was designed to circulate the headspace gas, so as to achieve a higher volumetric gas-liquid mass transfer coefficient (k(L)a). Periodic medium exchange was performed to avoid nutrient limitations. The CO utilization rate and ethanol productivity were maximized when the medium exchange ratio was held constant, and varied linearly with the gas circulation rate. The PBR operation using 2x medium composition resulted in maximum 16.1 g L-1 ethanol titer and 0.13 g L-1 h(-1) productivity. Consistent ethanol production was achieved throughout the reactor runs, confirming the stability of the PBR operation. The cost effectiveness of the PBR operation was determined based on the medium prices and the ethanol titer. The most realistic operation parameters were 0.01 h(-1) (mean dilution rate) and 1.5 vvm (gas circulation rate) using 12.6 times the nutrient reinforced medium.
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