Growth Inhibitory Effects of Antimicrobial Natural Products against Cariogenic and Health-Associated Oral Bacterial Species

Purpose: This study investigated whether selected natural products could specifically target the growth of a caries-associated bacterial species (Streptococcus mutans) without affecting the viability of a health-associated oral commensal bacterial species (Streptococcus sanguinis). Materials and Methods: Agar diffusion assays were used to screen the natural products for bacterial-growth inhibitory effects and the diameters of the inhibitory zones for the two bacterial species compared. The minimum inhibitory concentrations (MIC) of the natural products that showed growth inhibitory effects were determined using the broth microdilution method. Results: Except for the berry extracts (cranberry, wild blueberry, and strawberry), all the other selected natural products (peppermint, ginger, cinnamon, rosemary, liquorice, xanthorrrhizol, tt-farnesol, guaijaverin, and macelignan) exhibited varying degrees of bacterial growth inhibition. The MIC values ranged from as low as 4 mu g/ml for xanthorrrhizol to 1000 mu g/ml for guaijaverin. All the growth inhibitory natural agents tested showed similar inhibition for both S. mutans and S. sanguinis. Conclusions: Although several natural products exerted significant antibacterial effects, none had selective inhibitory action on the growth of S. mutans.