Journal
CYTOMETRY PART A
Volume 87A, Issue 6, Pages 568-579Publisher
WILEY
DOI: 10.1002/cyto.a.22629
Keywords
spatial statistics; colocalization; quantitative measurements; light microscopy; endocytosis
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Funding
- Agence Nationale de la Recherche [ANR-10-INBS-04-06 France-BioImaging, ANR-10-LABX-62-IBEID]
- Bourse Roux from Institut Pasteur
- Institut Pasteur [PTR 387]
- Institut pour la Recherche sur la Moelle et l'Encephale
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The quantitative analysis of molecule interactions in bioimaging is key for understanding the molecular orchestration of cellular processes and is generally achieved through the study of the spatial colocalization between the different populations of molecules. Colocalization methods are traditionally divided into pixel-based methods that measure global correlation coefficients from the overlap between pixel intensities in different color channels, and object-based methods that first segment molecule spots and then analyze their spatial distributions with second-order statistics. Here, we present a review of such colocalization methods and give a quantitative comparison of their relative merits in different types of biological applications and contexts. We show on synthetic and biological images that object-based methods are more robust statistically than pixel-based methods, and allow moreover to quantify accurately the number of colocalized molecules. (c) 2015 International Society for Advancement of Cytometry
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