Journal
ANALYST
Volume 145, Issue 6, Pages 2184-2190Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0an00035c
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Funding
- National Natural Science Foundation of China [21804103, 21805021]
- China Postdoctoral Science Foundation [2018M631057]
- Open Funding Project of Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) [ACBM2019009]
- General Program of Health and Family Planning Commission of Hubei Province of China [WJ2017M194]
- General Program of Natural Science Foundation of Hubei Province of China [2017CFB709]
- Frontier Project of Application Foundation of Wuhan Science and Technology Bureau of China [2018060401011314]
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The continuing hurdle of developing foodborne pathogen detection techniques is that compromises must be made among simplicity, portability, speed, sensitivity, and quantitation. Herein, we fabricated quantum dot nanobeads (QDNS) by a layer-by-layer assembly of quantum dots on the surface of polymer nanospheres. QDNS exhibited higher fluorescence intensity than the quantum dots at the same particle number. Based on the quantum dot nanobeads as the signal reporter, a quantitative lateral flow immunoassay was demonstrated for Salmonella typhimurium detection with improved sensitivity, specificity and accuracy. A visual detection limit of 5 x 10(3) CFU mL(-1)Salmonella typhimurium within 10 min has been proved and demonstrated. Additionally, higher concentrations of non-Salmonella typhimurium bacteria have negligible effects on the detection of Salmonella typhimurium. The results of 50 single blind tests by 10 testers suggested that the assay exhibited 100% accuracy. The results illustrate that the assay provides a balance among simplicity, speed, sensitivity and accuracy, and it can be a favorable alternative for Salmonella typhimurium screening in various samples.
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