3.8 Article

In-vitro antioxidant and antimicrobial activities of some varieties citrus grown in Algeria

Journal

ADVANCES IN TRADITIONAL MEDICINE
Volume 20, Issue 1, Pages 23-34

Publisher

SPRINGER
DOI: 10.1007/s13596-019-00379-9

Keywords

Antioxidant activity; Antimicrobial activity; Phenolic compounds; Citrus; Juice; Leaves

Funding

  1. Laboratory of Natural Products LAPRONA, Abou Bakr Belkaid University, Tlemcen, Algeria
  2. Natural Local Bio-resources Laboratory, Hassiba Benbouali University of Chlef
  3. Ministry of Higher Education and Scientific Research

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Polyphenols are scientifically researched molecules because of their multiple biological activities. This study aims to compare the phenolic content and estimate the antioxidant and anti-microbial activities of leaf extracts and juice of ten varieties Citrus fruits grown in Algeria. Antioxidant properties were evaluated by DPPH radical scavenging activity, beta-carotene-linoleic acid system and ferric reducing antioxidant capacity. Antimicrobial activity was carried out using agar well diffusion method. The results showed the highest levels of phenolic compounds in juice and leaves of different part and varieties of plant (*P<0.05). Among all the varieties, C. maxima and C. aurantium presents the highest activity for the three methods cited previously (94.44-91.66% and 95.41-83.84%). On the other hand, antibacterial activity present on the plates was indicated by an inhibition zone surrounding the well containing the juice. The C. Limon and C. aurantium varieties exhibited the highest antimicrobial activity, with inhibition zone ranging from (27.66 +/- 0.58, 24.66 +/- 1.53 mm) followed by grapefruit and mandarin with areas between (23.00 +/- 1.00, 17.66 +/- 0.58 mm) on the isolates used. In this study, whatever the parts of citrus (juice and leaves) have been found to contain higher amounts of phenolics and their efficiency as potent antioxidants and antimicrobial properties was confirmed.

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