Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 65, Issue 44, Pages 9693-9702Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b04552
Keywords
postharvest leaves; spreading; differentially expressed protein; gene expression; CsGOGAT protein; protein-protein interaction; tea plant
Funding
- National Natural Science Foundation of China [31570691]
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We analyzed the changes of theanine content in postharvest tea leaves under high temperature (38 degrees C), low temperature (4 degrees C), and shading spreadings by using ultrahigh-performance liquid chromatography. The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT, which are involved in theanine biosynthesis pathway, were identified from the corresponding proteome data. The protein protein interactions of CsFd-GOGAT and CsNADH-GOGAT, CsTS1, or CsNiR were verified by yeast two-hybrid technology. The expression profiles of 17 genes in theanine metabolism, including CsFd-GOGAT and CsNADH-GOGAT, were analyzed by quantitative real-time polymerase chain reaction. The correlations between the dynamic changes of theanine content and expression profiles of related genes and DEPs were analyzed. This study preliminarily proved the importance of CsGOGAT in dynamic changes of theanine content in postharvest tea leaves during spreading.
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