Novel Pyrimidine Tagged Silver Nanoparticle Based Fluorescent Immunoassay for the Detection of Pseudomonas aeruginosa

A simple pyrimidine-based fluorescent probe (R)-4-(anthracen-9-yl)-6-(naphthalen-1-yl)-1,6-dihydropyrimidine2-amine (ANDPA) was synthesized through the greener one pot reaction and characterized by IR, NMR, and ESI-Mass. Glucose stabilized silver nanoparticles (Glu-AgNPs) were also synthesized and characterized using UV, IR, XRD, SEM, and TEM. When ANDPA was tagged with Glu-AgNPs, the fluorescent intensity of ANDPA decreased drastically. When the monoclonal antibody (Ab) [immunoglobulin G (IgG)] of Pseudomonas aeruginosa (PA) was attached with ANDPA/Glu-AgNPs, the original intensity of the probe was recovered with minimal enhancement at 446 nm. On further attachment of PA with ANDPA/Glu-AgNPs/PA, the fluorescence intensity of the probe was enhanced obviously at 446 nm with red shift. This phenomenon was further supported by SEM and TEM. The linear range of detection is from 8 to 10(-1) CFU/mL, and LOD is 1.5 CFU/mL. The immunosensor was successfully demonstrated to detect Pseudomonas aeruginosa in water, soil, and food products like milk, sugar cane, and orange juices.