Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 65, Issue 29, Pages 6030-6041Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b02258
Keywords
sucrose phosphorylase; kojibiose; bulk functionality; Caco-2 cells; gut microbiota; fermentable sugars
Funding
- Fund for Scientific Research Flanders [S003617N]
- European Commission [265854]
- COST Chemistry CM1102 (MSMT project) [LD13042]
- Ghent University (Multidisciplinary Research Partnership Ghent Bio-Economy)
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Cost-efficient (bio)chemical production processes are essential to evaluate the commercial and industrial applications of promising carbohydrates and also are essential to ensure economically viable production processes. Here, the synthesis of the naturally occurring disaccharide kojibiose (2-O-alpha-D-glucopyranosyl-n-glucopyranoside) was evaluated using different Bifidobacterium adolescentis sucrose phosphorylase variants. Variant L341I_Q345S was found to efficiently synthesize kojibiose while remaining fully active after 1 week of incubation at 55 degrees C. Process optimization allowed kojibiose production at the kilogram scale, and simple but efficient downstream processing, using a yeast treatment and crystallization, resulted in more than 3 kg of highly pure crystalline kojibiose (99.8%). These amounts allowed a deeper characterization of its potential in food applications. It was found to have possible beneficial health effects, including delayed glucose release and potential to trigger SCFA production. Finally, we compared the bulk functionality of highly pure kojibiose to that of sucrose, hereby mapping its potential as a new sweetener in confectionery products.
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