Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 65, Issue 31, Pages 6506-6512Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b05457
Keywords
Olea europaea L. byproducts; hydroxytyrosol (HTyr); lipophilization of extracts; hydroxytyrosyl esters; colorectal cancer (CRC)
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A hydroxytyrosol (HTyr)-enriched fraction containing HTyr 6% w/w, derived from Olea europaea L. byproducts and obtained using an environmentally and economically sustainable technology, was lipophilized under green chemistry conditions. The effects of three fractions containing hydroxytyrosyl butanoate, octanoate, and oleate, named, respectively, lipophilic fractions 5, 6, and 7, and unreacted HTyr on the human colon cancer cell line HCT8-beta 8 engineered to overexpress estrogen receptor beta (ER beta) were evaluated and compared to those of pure HTyr. The experimental data demonstrated that HTyr and all fractions showed an antiproliferative effect, as had been observed by the evaluation of the cellular doubling time under these different conditions (mean control, 32 +/- 4 h; HTyr 1, 65 +/- 9 h; fraction 5, 64 +/- 11 h; fraction 6, 62 +/- 14 h; fraction 7, 133 +/- 30 h). As evidenced, fraction 7 containing hydroxytyrosyl oleate showed the highest activity. These results were related to the link with ER-beta, which was assessed through simultaneous treatment with an inhibitor of ER beta.
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