Journal
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
Volume 92, Issue 1, Pages -Publisher
ACAD BRASILEIRA DE CIENCIAS
DOI: 10.1590/0001-3765202020181203
Keywords
breast ductal carcinoma; plasma; miR-1273g-3p; circulating miRNA; biomarker
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Funding
- Scientific Research Project of Health and Family Planning Commission of Gansu Province, China [GSWSKY-2015-25]
- Natural Science Foundation for Young Scientists through Gansu provincial Science and Technology Department, China [17JR5RA042, 1606RJYA296]
- Gansu science and technology program through Gansu provincial Science and Technology Department, China [17JR3TA013]
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Circulating miRNAs presenting in plasma in a stable manner have been demonstrated their potential role as a promising biomarkers in many human diseases, such as Alzheimer's disease, melanoma and ovarian carcinoma. However, few circulating miRNAs could be used for breast ductal cancer diagnosis. Here, we identified miR-1273g-3p as a biomarker for detecting breast ductal cancer. We detected miR-1273g-3p levels in the plasma of 39 sporadic breast ductal cancer patients and 40 healthy donors by Stem-loop Quantitative Real-time PCR (qRT-PCR). The results showed the plasma miR-1273g-3p level were significantly up-regulated in breast ductal cancer patients compared with healthy donors (p=0.0139). Receiver operating characteristic (ROC) curve also revealed the significantly diagnostic ability of miR-1273g-3p in patients (p=0.0414). In addition, the plasma level of miR-1273g-3p was closely related to IIIB-IIIC TNM stage. We also confirmed the higher expression level of miR-1273g-3p in breast cancer cell lines MCF-7 (4.872 +/- 0.537) than normal breast cells (Hs 578Bst). Taken together, miR-1273g-3p could represent as a potential biomarker for early breast ductal cancer diagnosis.
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