Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 65, Issue 9, Pages 1952-1959Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.6b05574
Keywords
in vitro; prolamins; urea; sorghum; disulfide bonds
Funding
- U.S. Department of Agriculture-National Institute of Food and Agriculture [NEB-31-140]
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Proso millet protein has reported structural similarities with sorghum. In order to explore the potential of this crop as an alternative protein source for people with gluten sensitivity, in vitro protein digestibility was analyzed. Dehulled proso millet flour was subjected to various processing techniques (dry heating and wet heating). Regardless of the processing technique there was a significant decline in digestibility of protein in proso millet flour when compared with unprocessed flour (from 79.7 +/- 0.8% to 42.0 +/- 1.2%). Reduced digestibility persisted even when cooking with reducing agents. Heating in the presence of urea (8 M) and guanidine-HC1 (4.5 M) prevented the reduction in observed digestibility (urea cooked 77.4 +/- 0.8%; guanidine HC1 cooked 84.3 +/- 0.9%), suggesting formation of hydrophobic aggregates during heating in water. This was supported by an increase in surface hydrophobicity upon cooking. Thus, the proso millet protein, termed panicin, forms hydrophobic aggregates that are resistant to digestion when subjected to heat.
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