Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 65, Issue 44, Pages 9647-9654Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b03666
Keywords
osteoporosis; sophora flower; sophoridine; osteoclast differentiation; MAPK; NFATc1
Funding
- Natural Science Foundation of Qinghai [2016-ZJ-942Q]
- West Light Foundation of the Chinese Academy of Sciences [Y629071211]
- National Natural Science Foundation of China [81572123, 31701243]
- Science and Technology Commission of Shanghai Municipality [14431900900, 115411951100, 116430723500]
- Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support [20161314]
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An imbalance in osteogenesis and osteoclastogenesis is a crucial pathological factor in the development of osteoporosis. Osteoclasts (OCs) play a pivotal role in osteoporosis, whose new therapy exploration has been focused on the suppression of OC formation. Sophoridine is found from the Chinese traditional food sophora flower to exhibit anti-osteoporosis capacity by screening. This study is focused on its anti -osteoporosis mechanism evaluation. The anti -osteoporosis effect of sophoridine, (15 mg kg(-1) body), was evaluated in ovariectomized (OVX) mice by monitoring changes in bone histomorphometry index, formation of osteoclasts from blood-derived mononuclear cells, and changes in the synthesis of pro-osteodastogenic cytokines. Signal pathways were investigated by QPCR, Western blot, and immunofluorescence. Sophoridine has a significant anti-osteoporosis effect in vivo, which can inhibit RANKL-induced OC formation, the appearance of OC-specific marker genes, and OC marker protein in vitro. Mechanistically, sophoridine dose- and time-dependently blocks the RANKL-induced OC formation and the activation of ERK and c-Fos as well as the induction and nucleus translocation of NFATc1. Sophora flower might be a useful alternative functional food in preventing or treating osteoporosis.
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