Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 65, Issue 41, Pages 9094-9100Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b03405
Keywords
biodegradation; Pichia pastoris; organophosphorus hydrolase; extracellular expression; malathion
Funding
- National Key Research and Development Program of China [2016YFA0204300]
- National Natural Science Foundation of China [21505044, 21506055, 21536004]
- Shanghai Pujiang Program [15PJ1401200]
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The biodegradation of pesticides by organophosphorus hydrolases (OPHs) requires an efficient enzyme production technology in industry. Herein, a Pichia pastoris strain was constructed for the extracellular expression of PoOPHM9, an engineered malathion-degrading enzyme. After optimization, the maximum titer and yield of fermentation reached 50.8 kU/L and 4.1 g(protein)/L after 3 days, with the highest space-time yield (STY) reported so far, 640 U L-1 h(-1). PoOPHM9 displayed its high activity and stability in the presence of 0.1% (w/w) plant-derived detergent. Only 0.04 mg/mL enzyme could completely remove 0.15 mM malathion in aqueous solution within 20 min. Furthermore, 12 mu mol malathion on apples and cucumbers surfaces was completely removed by 0.05 mg/mL PoOPHM9 in tap water after 35 min washing. The efficient production of the highly active PoOPHM9 has cleared a major barrier to biodegradation of pesticide residues in food industry.
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