Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 65, Issue 20, Pages 4051-4056Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b00114
Keywords
arctigenin; 3'-desmethylarctigenin (3'-DMAG); 3'-desmethyl-4'-dehydroxyarctigenin (DMDH-AG); human intestinal bacteria; isolation; microbial biotransformation
Funding
- Applied and Fundamental Research Plan in Hebei Province of China (Key Basic Research Project) [16962504D]
- National Natural Science Foundation of China (NSFC) [31670057]
Ask authors/readers for more resources
Arctiin is the most abundant bioactive compound contained in the Arctium lappa plant. In our previous study, we isolated one single bacterium capable of bioconverting arctigenin, an aglycone of arctiin, to 3'-desmethylarctigenin (3'-DMAG) solely. However, to date, a specific bacterium capable of producing other arctiin metabolites has not been reported. In this study, we isolated one single bacterium, which we named Eggerthella sp. AUH-JLD49s, capable of bioconverting 3'-DMAG under anaerobic conditions. The metabolite of 3'-DMAG by strain AUH-JLD49s was identified as 3'-desmethyl-4'-dehydroxyarctigenin (DMDH-AG) based on electrospray ionization mass spectrometry (ESI-MS) and H-1 and C-13 nuclear magnetic resonance spectroscopy. The bioconversion kinetics and bioconversion capacity of strain AUH-JLD49s were investigated. In addition, the metabolite DMDH-AG showed an inhibitory effect on cell growth of human colon cancer cell line HCT116 and human breast cancer cell line MDA-MB-231.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available