Journal
FEMS MICROBIOLOGY LETTERS
Volume 367, Issue 12, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/femsle/fnaa092
Keywords
quorum-sensing; rsm system; virulence
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Funding
- Consejo Nacional de Ciencia y Tecnologia (CONACYT) [252269]
- Programa de Apoyo a Proyectos de Investigacion e Innovacion Tecnologica (PAPIIT) DGAPA, Universidad Nacional Autonoma de Mexico (UNAM) [IN201819, IA203519]
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Pseudomonas aeruginosa is an opportunistic human pathogen that is able to produce several virulence factors such as pyocyanin, rhamnolipids and elastase. In the clinical reference strain PAO1, synthesis of these virulence factors is regulated transcriptionally by quorum sensing (QS) and post-transcriptionally by the Rsm system. Herein, we investigated the role of these systems in the control of the pyocyanin, rhamnolipids and elastase production in the marine strain ID4365. We found that this strain carries a nonsense mutation in lasR that makes it a natural mutant in the Las QS system. However, its QS response is still functional with the Rh1 system activating virulence factors synthesis. We found that the Rsm system affects virulence factors production, since overexpression of RsmA reduces pyocyanin production whereas RsmY overexpression increases its synthesis. Unexpectedly, and in contrast to the type strain PAO1, inactivation of rsmA increases pyocyanin but reduces elastase and rhamnolipids production by a reduction of Rh1R levels. Thus, QS and Rsm systems are involved in regulating virulence factors production, but this regulation is different to the PAO1 strain even though their genomes are highly conserved. It is likely that these differences are related to the different ecological niches in which these strains lived.
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